APPLICATION OF RECOMBINANT SUCROSE SYNTHASE LARGE-SCALE SYNTHESIS OF ADP-GLUCOSE

Authors
ZERVOSEN A ROMER U ELLING L
Citation
A. Zervosen et al., APPLICATION OF RECOMBINANT SUCROSE SYNTHASE LARGE-SCALE SYNTHESIS OF ADP-GLUCOSE, Journal of molecular catalysis. B, Enzymatic, 5(1-4), 1998, pp. 25-28
Citations number
8
Categorie Soggetti
Chemistry Physical
Journal title
Journal of molecular catalysis. B, Enzymatic → ACNP
ISSN journal
13811177
Volume
5
Issue
1-4
Year of publication
1998
Pages
25 - 28
Database
ISI
SICI code
1381-1177(1998)5:1-4<25:AORSSL>2.0.ZU;2-U
Abstract
The synthesis of ADP-glucose with recombinant sucrose synthase from po tato was combined with the synthesis of ADP from AMP and ATP catalysed by myokinase from rabbit muscle. By using the repetitive-batch-techni que we were able to reach enzyme productivities (mg ADP-glucose/U enzy me) of 28 mg/U sucrose synthase and 140 mg/U myokinase yielding 2.8 g ADP-glucose (55% yield). After product isolation, 2.2 g ADP-glucose wa s obtained corresponding to 44% overall yield. (C) 1998 Elsevier Scien ce B.V. All rights reserved.