EVALUATION OF THE MACROCYCLIC ANTIBIOTIC AVOPARCIN AS A NEW CHIRAL SELECTOR FOR HPLC

Avoparcin is a macrocyclic glycopeptide antibiotic structurally relate d to vancomycin, teicoplanin, and ristocetin A. When attached to 5-mu m spherical silica gel, the avoparcin proved to be an effective chiral stationary phase (CSP) that could be used in the reversed-phase, norm al-phase, and polar-organic modes. The avoparcin CSP was complimentary to the other macrocyclic glycopeptide CSPs in that it could resolve s ome racemates that the others could not, and vice versa. Some importan t compounds resolved on the avoparcin CSP include verapamil, thyroxine , mephenytoin, and 2-imidazolidone-4-carboxylic acid. The use of this CSP and the optimization of separations on it are discussed. Avoparcin appears to be a useful addition to this family of CSPs. (C) 1998 Wile y-Liss, Inc.