HPLC OF AMINO-ACIDS USING TETRAPHENYLPORPHYRIN - SILICA STATIONARY PHASES

Authors
BIESAGA M ORSKA J TROJANOWICZ M
Citation
M. Biesaga et al., HPLC OF AMINO-ACIDS USING TETRAPHENYLPORPHYRIN - SILICA STATIONARY PHASES, Chemia Analityczna, 43(4), 1998, pp. 647-656
Citations number
23
Categorie Soggetti
Chemistry Analytical
Journal title
Chemia Analityczna → ACNP
ISSN journal
00092223
Volume
43
Issue
4
Year of publication
1998
Pages
647 - 656
Database
ISI
SICI code
0009-2223(1998)43:4<647:HOAUT->2.0.ZU;2-Y
Abstract
The retention of selected 18 amino acids was investigated on stationar y phases with immobilized unmetallated and metallated with Zn(II) and Cu(II) tetraphenylporphyrin. The effects of eluent composition, its co ncentration, pH, and the length of the column was examined. A mixed me chanism of retention was confirmed involving coordination of solute to metallic centres and hydrophobic interactions between aromatic rings of porphyrin and some amino acids.