THIN-LAYER CHROMATOGRAPHY AND ENZYMATIC ANALYSIS OF PHOSPHORORGANIC COMPOUNDS USING 4-METHYLUMBELLIFERONE ESTERS

The procedure of phosphororganic compounds analysis using thin layer c hromatography with enzymatic method of chromatograms visualization was elaborated. As the substrates of enzymatic reactions, using cholinest erase, esters of 4-methylumbelliferone were applied. The effects of re actions obtained when 4-methylumbelliferone esters were used were comp ared with the effects of reactions when indoxyl acetate was used. The performance of enzymatic reaction on a chromatographic plate was estim ated by the fluorescence measurement of the reaction product, which wa s 4-methylumbeliferone. The enzymatic reaction was performed by pressi ng the papers saturated with proper reagent to the chromatographic pla te. It was the way to eliminate from enzymatic reaction medium organic solvents used for dissolving 4-methylumbeliferone eaters. Using the p rocedure it was possible to determine sarin, soman and tabun at the le vel of dozen picograms per spot and VX as well as pesticides from the individual nanograms to their dozens per spot.