DEMONSTRATION OF FACILITATORY I-WAVE INTERACTION IN THE HUMAN MOTOR CORTEX BY PAIRED TRANSCRANIAL MAGNETIC STIMULATION

1. Transcranial magnetic stimulation (TMS) of the human motor cortex r esults in multiple discharges (D and I waves) in the corticospinal tra ct. We tested whether these volleys can be explored non-invasively wit h paired TMS. The intensity of the first stimulus (S1) was set to prod uce a motor-evoked potential (MEP) of 1 mV in the resting contralatera l abductor digiti minimi (ADM) muscle; the second stimulus (S2) was se t to 90% of the resting motor threshold. At interstimulus intervals of 1.1-1.5, 2.3-2.9 and 4.1-4.4 ms the MEP elicited by S1 plus S2 was la rger than that produced by S1 alone. 2. Varying the S1 intensity betwe en 70 and 130% resting motor threshold with S2 held constant at 90 % r esting motor threshold showed that the threshold for the first MEP pea k was less than or equal to 70% resting motor threshold. The second an d third MEP peaks appeared only at higher S1 intensities. The latency of all peaks decreased with increasing S1 intensity. 3. Varying the S2 intensity with S1 held constant to produce a MEP of 1 mV on its own s howed that the amplitude of all MEP peaks increased with S2 intensity, but that their timing remained unchanged. 4. Paired TMS in the active ADM (S1 clearly suprathreshold, SS just above threshold; interstimulu s interval, 1 ms) produced strong MEP facilitation. The onset of this facilitation occurred later by about 1.5 ms than the onset of the MEP evoked by S2 alone. No MEP facilitation was seen if the magnetic S2 wa s replaced by anodal or cathodal transcranial electrical stimulation. 5. It is concluded that the MEP facilitation after paired TMS, at leas t for the first MEP peak, is due to facilitatory interaction between I waves, and takes place in the motor cortex at or upstream from the co rticospinal neurone.