EFFECT OF POSTINDUCTION NUTRIENT FEEDING STRATEGIES ON THE PRODUCTIONOF BIOADHESIVE PROTEIN IN ESCHERICHIA-COLI

The effect of post-induction nutrient feeding strategies on the produc tion of bioadhesive protein using an IPTG inducible expression system in Escherichia coil was investigated. Cells were cultured in an expone ntial fed-batch mode to the OD600 of ca. 100 (48 gDCW/L) prior to indu ction. Six different post-induction nutrient feeding strategies (pH-st at, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein producti on was examined. It was found that post-induction cell growth was inde pendent of nutrient feeding rate. However, bioadhesive protein product ion was significantly affected by post-induction feeding strategies. L inearly changing post-induction feeding rate with a suitable slope all owed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies. (C ) 1998 John Wiley & Sons, Inc.