AGONIST STIMULATION OF B-1 AND B-2 KININ RECEPTORS CAUSES ACTIVATION OF THE MAP KINASE SIGNALING PATHWAY, RESULTING IN THE TRANSLOCATION OFAP-1 IN HEK-293 CELLS

In response to bradykinin, phosphorylated MAP kinases (ERK-1 and ERK-2 ) were abundantly increased in HEK 293 cells, which overexpress the ra t B-2 kinin receptor. In a similar may des-Arg(9)-bradykinin stimulati on of B-1 kinin receptor-overexpressing HEK 293 cells caused activatio n of the same species of MAP kinase, Furthermore, nuclear translocatio n of transcription factor AP-1 was also found in the cells after stimu lation with either agonist, PD98059, a MAP kinase kinase (MEK-1) inhib itor, blocked the agonist-induced AP-1 translocation as well as the ph osphorylation of the MAP kinases, This communication provides the firs t evidence for both B-1 and B-2 kinin receptors mediating the MAP kina se signaling pathway to activate AP-1, (C) 1998 Federation of European Biochemical Societies.