INHIBITION OF MALONALDEHYDE AND ACETALDEHYDE FORMATION FROM BLOOD-PLASMA OXIDATION BY NATURALLY-OCCURRING ANTIOXIDANTS

The inhibitory effect of 2''-O-glycosylisovitexin (2''-O-GIV), 2,5-dim ethyl-4-hydroxy-3(2H)-furanone (DMHF), L-ascorbic acid (vitamin C), an d 4,4'-(isopropylidenedithio)bis(2,6-di-tert-butyl phenol) (probucol) on malonaldehyde (MA) and acetaldehyde formation from horse blood plas ma oxidized with Fenton's reagent was determined by gas chromatography . 2''-O-GIV, DMHF, and L-ascorbic acid inhibited MA formation at a lev el of 100 nmol by 60, 22, and 22%, respectively; probucol did not show any inhibitory activity on MA formation. Improved inhibitory activity toward MA formation was observed when 2''-O-GIV or DMHF was mixed wit h L-ascorbic acid in equal amounts. Approximately 80-90% of acetaldehy de formation was inhibited by 300 nmol of 2''-O-GIV and probucol, wher eas DMHF required 1000 nmol to exhibit the same level of inhibition.