D. Lucas et al., COMPARISON OF LEVELS OF CYTOCHROMES-P-450, CYP1A2, CYP2E1, AND THEIR RELATED MONOOXYGENASE ACTIVITIES IN HUMAN SURGICAL LIVER SAMPLES, Alcoholism, clinical and experimental research, 17(4), 1993, pp. 900-905
Hepatic microsomal cytochromes P-450 CYP1A2, and CYP2E1 contents and c
atalytic activities have been simultaneously investigated in 42 patien
ts undergoing diagnostic liver biopsy. CYP1A2 contents, measured by We
stern blotting, were correlated with methoxyresorufin-O-demethylation
and ethoxyresorufin-O-deethylation (r = 0.65 and r = 0.66, p < 0.001,
respectively). CYP2E1 contents were correlated with 1-butanol oxidatio
n and 6-hydroxylation of chlorzoxazone (r = 0.75 for both, p < 0.001).
CYP1A2 catalytic activities varied by 30- to 40-fold, whereas CYP2E1
activities varied by 6- to 20-fold. In our study, these variations wer
e not related to liver diseases or cancer of the digestive tract nor t
o alcohol drinking or smoking habits, because patients were alcohol- a
nd tobacco-free for 1 month before the study. Other environmental fact
ors, diet habits, and/or genetic factors could explain the large inter
individual variations observed.