R. Tunga et al., OPTIMIZING SOME FACTORS AFFECTING PROTEASE PRODUCTION UNDER SOLID-STATE FERMENTATION, Bioprocess engineering, 19(3), 1998, pp. 187-190
Production of extracellular alkaline protease by a locally isolated fu
ngal species, Rhizopus oryzae, under solid state fermentation was opti
mized. The maximum enzyme activity under the optimum conditions of tem
perature (32 degrees C), relative humidity (90%-95%), spore count (sim
ilar to 2 X 10(5)/g wheat bran), moisture content of solid substrate (
140%) adjusted suitably with salt solution (M-9) of pH 5.5 was 341 uni
t/g wheat bran.