S. Ananthan et P. Saravanan, ANALYSIS OF HUMAN ROTAVIRUS SEROTYPES IN CHILDREN WITH ACUTE DIARRHEAIN CHENNAI BY MONOCLONAL-ANTIBODY BASED ELISA, INDIAN JOURNAL OF MEDICAL RESEARCH, 108, 1998, pp. 58-61
Citations number
30
Categorie Soggetti
Medicine, General & Internal","Medicine, Research & Experimental",Immunology
To determine the individual human rotavirus serotypes prevailing in Ch
ennni, 345 stool specimens obtained from children with acute diarrhoea
between March 1996 and November 1997, were screened for the presence
of rotavirus by the standardized enzyme linked immunosorbent assay (EL
ISA). Of the 90 (26%) rotavirus positive specimens, 75 (83.3%) were su
bgrouped and 65 (72.2%) were serotyped with monoclonal antibody based
ELISA. Of the 65 specimens that could be serotyped, 52.3 per cent belo
nged to serotype 2, 24.6 per cent were serotype 4, 15.4 per cent were
serotype 1 and 7.7 per cent were serotype 3. Of the 75 specimens typed
for their subgroup specificity, 50.7 per cent were subgroup I, 26.7 p
er cent were subgroup II, 18.7 per cent were equally specific to both
subgroups I and II, 4 per cent belonged to nonsubgroups I and II. Our
results indicate a predominance of serotype 2 virus. Unusual strain ha
ving both subgroups I and II specificity or neither specificity were a
lso encountered.