MURINE TROPHOBLAST FAILURE AND SPONTANEOUS-ABORTION

PROBLEM: Infection has been proposed to initiate abortion, and the rol e of viruses in spontaneous resorption in mice has not been tested. ME THOD: The anti-viral drug ribavirin ta-D-ribofuranosyl-1,2,4-triazole- 3-3-carboxamide) was fed to CBA/J and C3H/HeJ female mice beginning on the morning after mating with DBA/2J males. RESULTS: Ribavirin treatm ent increased the rate of abortion (resorption) on day 13.5, and this was associated with retardation of the rate of embryo development and hypoplasia of the trophoblast. There was a reduction in trophoblast-de pendent decidua-associated soluble suppressor activity, but there was no maternal mononuclear cell infiltrate of the type reported in associ ation with resorption of semiallogeneic and xenogeneic mouse embryos. This may be due to an immunosuppressive effect of ribavirin. Ribavirin was able to potently suppress proliferation of mouse trophoblast and mastocytoma cell lines in vitro. CONCLUSIONS: There are several drug-i nduced murine abortion models that provide useful insights into potent ial mechanisms underlying spontaneous pregnancy failure, but in the ri bavirin model, a direct impairment of trophoblast development appears to be responsible.