RAPID AND HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF HISTAMINE AND 3-METHYLHISTAMINE IN BIOLOGICAL SAMPLES USING FLUORESCAMINE AS THE DERIVATIZING AGENT

Authors
VANHAASTER CMCJ ENGELS W LEMMENS PJMR HORNSTRA G VANDERVUSSE GJ
Citation
Cmcj. Vanhaaster et al., RAPID AND HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF HISTAMINE AND 3-METHYLHISTAMINE IN BIOLOGICAL SAMPLES USING FLUORESCAMINE AS THE DERIVATIZING AGENT, Journal of chromatography. Biomedical applications, 617(2), 1993, pp. 233-240
Citations number
24
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
617
Issue
2
Year of publication
1993
Pages
233 - 240
Database
ISI
SICI code
0378-4347(1993)617:2<233:RAHSHL>2.0.ZU;2-I
Abstract
A highly sensitive and rapid high-performance liquid chromatographic a ssay for the determination of histamine and 3-methyl-histamine in biol ogical samples using 1-methylhistamine as the internal standard is des cribed. Samples were purified and concentrated on cation-exchange colu mns and derivatized with fluorescamine. The lower detection limit was 20 pg on-column. Linearity was demonstrated up to 20 ng on-column. The samples could be derivatized simultaneously before injection and were stable for 7 days. The method was used for the determination of hista mine and related compounds in coronary perfusates, extracts of homogen ized rat hearts, and supernatants of stimulated peritoneal mast cells.