RAPID AND HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF HISTAMINE AND 3-METHYLHISTAMINE IN BIOLOGICAL SAMPLES USING FLUORESCAMINE AS THE DERIVATIZING AGENT
Cmcj. Vanhaaster et al., RAPID AND HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF HISTAMINE AND 3-METHYLHISTAMINE IN BIOLOGICAL SAMPLES USING FLUORESCAMINE AS THE DERIVATIZING AGENT, Journal of chromatography. Biomedical applications, 617(2), 1993, pp. 233-240
A highly sensitive and rapid high-performance liquid chromatographic a
ssay for the determination of histamine and 3-methyl-histamine in biol
ogical samples using 1-methylhistamine as the internal standard is des
cribed. Samples were purified and concentrated on cation-exchange colu
mns and derivatized with fluorescamine. The lower detection limit was
20 pg on-column. Linearity was demonstrated up to 20 ng on-column. The
samples could be derivatized simultaneously before injection and were
stable for 7 days. The method was used for the determination of hista
mine and related compounds in coronary perfusates, extracts of homogen
ized rat hearts, and supernatants of stimulated peritoneal mast cells.