IDENTIFICATION OF MOUSE CRYSTALLINS IN 2D PROTEIN-PATTERNS BY SEQUENCING AND MASS-SPECTROMETRY - APPLICATION TO CATARACT MUTANTS

The eye lens proteins of the mouse were separated into 1940 polypeptid e spots by two-dimensional electrophoresis in large gels. All 16 cryst allins ubiquitous in mammals were identified by protein sequencing and mass spectrometry except for (gamma)-F, which shows an almost identic al sequence with (gamma)-E, Two crystallins, (beta)-A2 and (gamma)-S, were shown for the first time to occur in the mouse lens, An investiga tion of the murine cataract mutant Cat2(nop) ((gamma)-B gene) demonstr ated that a monogenic mutation might affect a broad spectrum of protei ns, (C) 1998 Federation of European Biochemical Societies.