GROWTH-FACTOR PROTECTION AGAINST CYTOKINE-INDUCED APOPTOSIS IN NEONATAL RAT ISLETS OF LANGERHANS - ROLE OF FAS

Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1 beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M , interferon-gamma 5 U/ml) led to extensive cell death, which was pote ntiated by Fas activation with the anti-Fas cytolytic antibody JO2, Pr e-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (1 0(-8) M) gave only partial protection against cell killing, but preven ted the Fas-mediated component, In the absence of cytokine treatment, Fas-mediated killing was not observed. (C) 1998 Federation of European Biochemical Societies.