BIPHASIC INCREASE IN SERUM INHIBIN-B DURING PUBERTY - A LONGITUDINAL-STUDY OF HEALTHY FINNISH BOYS

We investigated the concentrations of serum inhibin B and the pro-alph a C-containing inhibins (pro-alpha C inhibin) and their relations to s erum FSH levels in 38 healthy boys during their progression through pu berty. Furthermore, we studied the effect of recombinant human FSH (rh FSH) on pro-alpha C inhibin production in three prepubertal gonadotrop in-deficient boys. The serum inhibin B level increased between Tanner stages G1 and G2, (p < 0.02), simultaneously with the serum LH and tes tosterone concentrations. In advanced puberty, inhibin B changed less, and at stage G4, correlated negatively with serum FSH level (r = -0.5 7, p < 0.001, n = 37). The serum pro-alpha C inhibin level changed dif ferently and increased also in advanced puberty. In prepubertal health y subjects, the serum pro-alpha C inhibin and FSH levels correlated po sitively (r = 0.50, p = 0.051, n = 16), and during the rhFSH treatment of gonadotropin-deficient bays, serum pro-alpha C inhibin levels incr eased. These findings suggest that an increased production of inhibin B is an early event in puberty and that inhibin B can inhibit pituitar y FSH secretion in the course of sexual maturation. Furthermore, they suggest that during puberty free inhibin (pro)-alpha-subunits, detecte d by the pro-alpha C inhibin assay, are present in large amounts and t hat FSH stimulates their production by prepubertal testes.