C. Bamdad, A DNA SELF-ASSEMBLED MONOLAYER FOR THE SPECIFIC ATTACHMENT OF UNMODIFIED DOUBLE-STRANDED-DNA OR SINGLE-STRANDED-DNA, Biophysical journal, 75(4), 1998, pp. 1997-2003
A novel method for DNA surface immobilization and a paradigm for the a
ttachment of unmodified DNA of any length or sequence are described he
rein. The development of a DNA self-assembled monolayer (DNA-SAM) that
incorporates a DNA-thiol into a monolayer of inert alkane thiolates i
s reported. This DNA-SAM specifically hybridized complementary oligonu
cleotides while resisting the nonspecific adsorption of noncomplementa
ry DNA and irrelevant proteins. Duplex DNA, having a single-stranded '
'capture tail,'' specifically bound to the DNA-SAM if the sequence of
the ''tail'' was complementary to DNA presented in the SAM. The sense
strand of the hybridized duplex DNA could be covalently attached to th
e surface by an enzymatic ligation reaction (leaving the anti-sense st
rand dissociable). DNA-binding proteins specifically bound to these su
rfaces only if their cognate sites were present in the duplex DNA.