SYNTHESIS AND FUNCTIONS OF A GLYCOPOLYMER CARRYING GAL-BETA-1-]4(GLCNAC)(3) TETRASACCHARIDE

Authors
KOBAYASHI K KAMIYA S MATSUYAMA M MURATA T USUI T
Citation
K. Kobayashi et al., SYNTHESIS AND FUNCTIONS OF A GLYCOPOLYMER CARRYING GAL-BETA-1-]4(GLCNAC)(3) TETRASACCHARIDE, Polymer Journal, 30(8), 1998, pp. 653-658
Citations number
30
Categorie Soggetti
Polymer Sciences
Journal title
Polymer Journal → ACNP
ISSN journal
00323896
Volume
30
Issue
8
Year of publication
1998
Pages
653 - 658
Database
ISI
SICI code
0032-3896(1998)30:8<653:SAFOAG>2.0.ZU;2-Y
Abstract
Tetrasaccharide Gal beta 1-->4(GlcNAc), was synthesized from N,N',N '' -triacetylchitotriose (GlcNAc), and lactose using transglycosylation w ith a beta-D-galactosidase from Bacillus circulans. The reducing termi nal of Gal beta 1-->4(GlcNAc)(3) was oxidized and connected to p-vinyl benzylamine Pia amide linkage, and the resulting oligosaccharide-subst ituted styrene monomer was polymerized with the radical initiator, 2,2 '-azobis(2-amidinopropane) dihydrochloride at 60 degrees C. Glycopolys tyrene was found to bind strongly with wheat germ agglutinin (WGA) and tomato (Lycopersicon esculentum) agglutinin (LEA) by inhibition of he magglutination and double diffusion.