EVALUATION OF PARENCHYMAL AND NONPARENCHYMAL CELL INJURY AFTER DIFFERENT CONDITIONS OF STORAGE AND REPERFUSION

We used the isolated perfused rat liver model (IPRL) to assess parench ymal and nonparenchymal cell integrity after different conditions of s torage and reperfusion. Two studies were performed. In study I, the IP RL was applied to evaluate the effects of 30 min of normothermic reper fusion with Elohes solution, enriched William's medium (Wif), or Carol ina rinse solution (CRS) following 24 h of cold preservation in high-K + or high-Na+ UW solution. As indicated by creatine kinase-BB (CK-BB) release, reperfusion with CRS provided greater protection of endotheli al cells after storage in high-K+ UW solution than after storage in hi gh-Na+ UW solution. In study 2, livers were cold-preserved (24 h, 4 de grees C) in either high-K+ or high-Na+ UW solution, then flushed with either CRS or Wif solution at room temperature before reperfusion (120 min, 37 degrees C) with 5% albumin-William's medium E. There was no s tatistical difference between the rinse solutions for bile flow and tr ansaminases release. However, CRS improved bile indocyanine green excr etion, which is known to be a marker of parenchymal and nonparenchymal cell integrity. Therefore, we can assume that this rinse solution pro tects rat liver grafts from reperfusion-induced microvascular damage.