CHARACTERIZATION OF AN ECHOVIRUS TYPE-11' (PRIME) EPIDEMIC STRAIN CAUSING HEMORRHAGIC SYNDROME IN NEWBORN BABIES IN HUNGARY

Citation
Mm. Elsageyer et al., CHARACTERIZATION OF AN ECHOVIRUS TYPE-11' (PRIME) EPIDEMIC STRAIN CAUSING HEMORRHAGIC SYNDROME IN NEWBORN BABIES IN HUNGARY, Acta virologica (Anglicka verze), 42(3), 1998, pp. 157-166
Citations number
70
Categorie Soggetti
Virology
ISSN journal
0001723X
Volume
42
Issue
3
Year of publication
1998
Pages
157 - 166
Database
ISI
SICI code
0001-723X(1998)42:3<157:COAET(>2.0.ZU;2-U
Abstract
Echovirus 11' (prime) isolates from an epidemic of haemorrhagic syndro me in departments of obstetrics in Hungary have been characterised. Th e leading component of the clinical disease was carditis and its letha l outcome occurred in 13 newborn babies. Maternal immunity was found t o be absent even in women of 41 years of age. The application of monov alent oral poliovirus type I vaccine prevented the progress of the epi demic within two weeks. Nevertheless, a serological survey among primo vacinees of 3-15 months of age revealed that 20% of the babies serocon verted without clinical symptoms during the epidemic. Serological evid ence showed that the echovirus 11' infection was unable to interfere w ith the efficacy of oral poliovirus vaccine (OPV), since seroconversio n rates of primovaccinees did not differ significantly from those in t he group seroconverted also to echovirus 11' during the vaccination ca mpaign. A 440 nucleotide (nt) fragment of the 5'-non-translated region of 12 epidemic echovirus 11' isolates and 26 echovirus prototype stra ins was amplified by a nested reverse transcription-polymerase chain r eaction (RT-PCR) and analysed using three different restriction endonu cleases. The 5'-regions of the echovirus 11' isolates were found to be identical to each other but different from that of the prototype echo virus 11 (Gregory) strain. The results indicate that echovirus 11' iso lates underwent genetic changes in the 5'-end and P1 region of the gen ome before the onset of the epidemic.