IN-VITRO ACTIVITIES OF AMPHOTERICIN-B, ITRACONAZOLE AND VORICONAZOLE AGAINST 150 CLINICAL AND ENVIRONMENTAL ASPERGILLUS-FUMIGATUS ISOLATES

The in-vitro activity of amphotericin B, itraconazole and voriconazole against 130 clinical and 20 environmental Aspergillus fumigatus isola tes was tested with an agar dilution method using RPMI 1640 medium. It raconazole-susceptible (AF71) and -resistant (AF90) isolates were incl uded in each test and all isolates were tested in duplicate. Geometric mean MIC values (ranges) at 48 h and 72 h were, respectively, amphote ricin B, 0.91 (0.25-2) and 1.50 (1-4) mg/L; itraconazole, 0.25 (0.06-1 ) and 0.48 (0.125-8) mg/L; and voriconazole, 0.56 (0.25-8) and 0.78 (0 .25-8) mg/L. The reproducibility of the results was high for all drugs . In-vitro resistance of A. fumigatus to the tested antifungal agents was uncommon and the MICs of itraconazole were half those of voriconaz ole (P < 0.01).