ENZYMATIC TRANSFORMATION OF CEPHALOSPORIN-C TO 7-ACA BY SIMULTANEOUS ACTION OF IMMOBILIZED D-AMINO-ACID OXIDASE AND GLUTARYL-7-ACA ACYLASE

The enzymatic transformation of cephalosporin C (CEPH C) to 7-amino-ce phalosporanic acid (7-ACA) using D-amino acid oxidase (DAO) and glutar yl-7-ACA acylase (Gl-7-ACA) is reported. The enzymes have been immobil ized separately on different carriers, in order to maximize the cataly tic activity and the stability. The reaction has been carried out in s ingle-step-like conditions, using the two enzymes simultaneously The e ffect of catalase contamination, present in the DAO preparations, was balanced by addition of extra hydrogen peroxide. In optimum conditions , the conversion of CEPH C to 7-ACA was higher than 90%, with byproduc t formation lower than 4%. The mixture of immobilized enzymes was reus ed in repeated reaction cycles, showing an appreciable operational sta bility.