ENZYMATIC DETERMINATION OF HYDRAZONIUM SALTS OF DIALKYLDITHIOPHOSPHORIC ACIDS

The analytical characteristics of procedures for the determination of dimethylhydrazone salts of benzaldehyde and cinnamaldehyde derivatives and dialkyldithiophosphoric acids using native and paper-immobilized butyryl cholinesterase were examined. It was found that, depending on the analytical conditions, the above compounds can exhibit both revers ible and irreversible inhibiting effects due to hydrazonium and thioph osphate ions, respectively. Methods were proposed for decreasing the d etection limit, including electrochemical activation and suppressing t he nonspecific sorption of inhibitors by adding surfactants.