Soybean [Glycine max (L.) Merr.] seed with yellow seed coat and hilum
is preferred in North America for the export market. Seed coats may da
rken or discolor when exposed to chilling temperatures during seed dev
elopment. We examined the effects of chilling temperatures on seed coa
t discoloration of yellow hilum, isogenic lines of 'Maple Presto' soyb
ean (Maturity Group 000) differing in pubescence color (I, r, T, tawny
; I, r, t, gray). The isogenic lines were grown in the field for five
seasons and once in the growth cabinet (25/20 degrees C day/night). We
quantified seed coat discoloration using an image analyzer. When expo
sed to cool temperatures during seed development, the seed coat darken
ed. In the extreme case, the seed coat from the taw ny line looked gra
y-brown. The tawny line discolored more than the gray line, both in th
e field and the growth cabinet. Seed coat discoloration increased with
the accumulation of daily minimum temperature less than or equal to 1
5 degrees C during seed development. In years with cool temperatures d
uring seed development, seed coats may discolor, reducing the quality
of the seed's appearance.