ESTABLISHMENT OF MONOCLONAL-ANTIBODY AGAINST HUMAN APO B-48 AND MEASUREMENT OF APO B-48 IN SERUM BY ELISA METHOD

The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is tr anslated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide representing human Apo B-48 C-t erminal sequence as immunogen, we established a monoclonal antibody, B 48-151, against human Apo B-48. The specific reactivity for Apo B-48 o f this monoclonal antibody was confirmed using Western blot analysis o f human plasma in fractions isolated as chylomicron and VLDL. Then, we developed a simple sandwich ELISA method for the detection of human A po B-48 in serum by combining B48-151 as capturing antibody and HRP-co njugated-polyclonaI antibodies for Apo B as signaling antibody. The es tablished sandwich ELISA constitutes a simple method to monitor Apo B- 48 level in chylomicrons and chylomicron remnants in human serum. J. C lin. Lab. Anal. 12:289-292, 1998. (C) 1998 Wiley-Liss, Inc.