RAPID ANALYSIS OF GUANIDINO COMPOUNDS IN SERUM FROM NEPHRITIC PATIENTS USING COLUMN-SWITCHING WITH ISOCRATIC ELUTION

The rapid method far baseline separation of ten guanidino compounds in serum from nephritic patients was designed using a single eluent with a column switching system. A porous graphitic carbon column and an oc tadecyl-bonded silica gel columns were used, (50 mm x 4.6 mm i.d.). Se paration was completed within 15 min. The stable baseline permitted hi ghly sensitive detection with excellent reproducibility. The system wa s applied to analyze guanidino compounds in sera from 175 nephritic pa tients. The hemodialysis process could not completely eliminate guanid ino compounds, and the degree of removal varied between patients. The correlation among metabolites indicated the differences in disease. (C ) 1998 John Wiley & Sons, Ltd.