EFFECT OF PHOSPHATIDYLSERINE ON FREE-RADICAL SUSCEPTIBILITY IN HUMAN-DIPLOID FIBROBLASTS

We studied the effect of phosphatidylserine (PdtSER) on oxygen metabol ite toxicity in skin fibroblast cell lines from apparently normal subj ects. Fibroblast damage was produced by the generation of oxygen metab olites during the enzymatic oxidation of acetaldehyde by xanthine-oxid ase (Xo). In order to quantify cell damage, we measured lactate dehydr ogenase (LDH) activity in culture medium and cell viability in fibrobl ast cultures, with and without preincubation for 4 days with PdtSER 13 muM, after Xo incubation. We found a significant increase of LDH acti vity in culture medium of cells without preincubation with PdtSER. No significant increase of LDH activity was observed in the same cell lin es after preincubation with PdtSER.