S. Latorraca et al., EFFECT OF PHOSPHATIDYLSERINE ON FREE-RADICAL SUSCEPTIBILITY IN HUMAN-DIPLOID FIBROBLASTS, Journal of neural transmission. Parkinson's disease and dementia section, 6(1), 1993, pp. 73-77
We studied the effect of phosphatidylserine (PdtSER) on oxygen metabol
ite toxicity in skin fibroblast cell lines from apparently normal subj
ects. Fibroblast damage was produced by the generation of oxygen metab
olites during the enzymatic oxidation of acetaldehyde by xanthine-oxid
ase (Xo). In order to quantify cell damage, we measured lactate dehydr
ogenase (LDH) activity in culture medium and cell viability in fibrobl
ast cultures, with and without preincubation for 4 days with PdtSER 13
muM, after Xo incubation. We found a significant increase of LDH acti
vity in culture medium of cells without preincubation with PdtSER. No
significant increase of LDH activity was observed in the same cell lin
es after preincubation with PdtSER.