PRODUCTION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HYPODERMIN-A, A SERINE-PROTEASE OF THE COMMON CATTLE GRUB, HYPODERMA-LINEATUM

Murine hybridomas were constructed that secreted monoclonal antibodies specific for hypodermin A (HyA), a serine protease produced by first- instar larvae of the common cattle grub, Hypoderma lineatum (Villers). Monoclonal antibodies produced by the three hybridoma cultures, HL1-A 1, HL1-A2 and HL1-A3 were each of subclass IgG1. HL1-A1 antibody bound only the native form of HyA, while HL1-A2 and HL1-A3 antibodies bound both native and SDS-denatured forms of HyA. Binding of either HL1-A1, HL1-A2 or HL1-A3 monoclonal antibodies to HyA hindered subsequent bin ding of any of the other monoclonal antibodies. HL1-A1 monoclonal anti bodies were purified and utilized to develop a sandwich ELISA capable of quantitation of HyA from natural or recombinant sources.