G. Kampf et al., DIRECT-DETECTION OF PNEUMOCYSTIS-CARINII IN OUTDOOR AIR - PRELIMINARY-RESULTS OF A FIELD-STUDY, Zentralblatt fur Hygiene und Umweltmedizin, 201(3), 1998, pp. 241-249
P. carinii causes pneumonia in immunocompromised patients. New studies
are suggestive of de novo infections. Although P. carinii is believed
to be ubiquitous it has not been detected directly from outdoor air.
Therefore, outdoor air samples (n = 20) of 5 m(3) were collected onto
gelatin filters (pore size: 3 mu m) on a cemetery using a suction pump
(Sartorius MD8). Five samples were silver-stained, five samples were
stained with an indirect immunfluorescence test kit, the remaining ten
samples were stained with the combinations of the two individual stai
ns. In a total of 100 m(3) outdoor air one cyst resembling P. carinii
was detected by the methenamine silver stain. Other cells nor resembli
ng P. carinii were found with a silver stained cell wall and positive
fluorescence. The natural reservoir of P. carinii remains unknown. The
indirect immunfluorescence which is common in the clinical laboratory
is probably not specific enough for environmental samples.