Kc. Lewis et al., CAPILLARY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC)WITH ELECTROSPRAY CONDENSATION PARTICLE COUNTING DETECTION, The Journal of microcolumn separations, 10(6), 1998, pp. 467-471
Protein standards are separated on a reverse-phase liquid chromatograp
hy column with 40 mu m inner diameter. They are introduced to the gas
phase by micro-electrospray and detected by a condensation particle co
unter. The micro-electrospray needle is a new design which allows for
the use of a nanoliter-per-minute makeup flow instead of the conventio
nal microliter-per-minute flow. The observed separation efficiency is
very good and the sheath micro-electrospray needle performs flawlessly
while the system detection limit is comparable to on-column UV absorp
tion. (C) 1998 John Wiley & Sons, Inc.