CAPILLARY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC)WITH ELECTROSPRAY CONDENSATION PARTICLE COUNTING DETECTION

Protein standards are separated on a reverse-phase liquid chromatograp hy column with 40 mu m inner diameter. They are introduced to the gas phase by micro-electrospray and detected by a condensation particle co unter. The micro-electrospray needle is a new design which allows for the use of a nanoliter-per-minute makeup flow instead of the conventio nal microliter-per-minute flow. The observed separation efficiency is very good and the sheath micro-electrospray needle performs flawlessly while the system detection limit is comparable to on-column UV absorp tion. (C) 1998 John Wiley & Sons, Inc.