COLOCALIZATION OF 5-HT1B-RECEPTOR AND 5-HT1D-RECEPTOR MESSENGER-RNA IN SEROTONERGIC CELL-BODIES IN GUINEA-PIG DORSAL RAPHE NUCLEUS - A DOUBLE-LABELING IN-SITU HYBRIDIZATION HISTOCHEMISTRY STUDY

In order to provide further details on the cellular localization of 5- HT1B- and 5-HT1D receptor mRNA in the dorsal raphe nucleus, we perform ed, in the same sections of guinea-pig dorsal raphe nucleus, double la beling in situ hybridization histochemistry for: (1) 5-HT1B receptor m RNA and 5-HT1D receptor mRNA, (2) 5-HT1B receptor mRNA and 5-HT transp orter (5-HTT) mRNA as marker for serotonergic neurons and (3) 5-HT1D r eceptor mRNA and 5-HTT mRNA. The 5-HT1B receptor mRNA was present in a ll cells containing 5-HT1D receptor mRNA. Similarly, both 5-HT1B- and 5-HT1D receptor mRNA was present in all 5-HTT mRNA positive cells. The present study demonstrates that 5-HT1B- and 5-HT1D receptor mRNA is c o-localized in serotonergic cell bodies of the guinea pig dorsal raphe nucleus. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.