GROWTH-FACTORS AND COMPONENTS FOR EXTRACELLULAR PROTEOLYSIS ARE DIFFERENTIALLY EXPRESSED DURING IN-VITRO MATURATION OF BOVINE CUMULUS-OOCYTE COMPLEXES

Citation
B. Bieser et al., GROWTH-FACTORS AND COMPONENTS FOR EXTRACELLULAR PROTEOLYSIS ARE DIFFERENTIALLY EXPRESSED DURING IN-VITRO MATURATION OF BOVINE CUMULUS-OOCYTE COMPLEXES, Biology of reproduction, 59(4), 1998, pp. 801-806
Citations number
35
Categorie Soggetti
Reproductive Biology
Journal title
ISSN journal
00063363
Volume
59
Issue
4
Year of publication
1998
Pages
801 - 806
Database
ISI
SICI code
0006-3363(1998)59:4<801:GACFEP>2.0.ZU;2-K
Abstract
In the present study, the time-dependent collagenolytic potential and mRNA transcription of extracellular matrix (ECM)-degrading components, transforming growth factor beta 1 (TGF beta 1), and both basic fibrob last growth factor (bFGF) and FGF receptors (FGFR) in bovine cumulus-o ocyte complexes (COCs) were investigated during 24 h of in vitro matur ation (IVM). COCs were collected from 2- to 6-mm follicles, cultured i n maturation medium, and sequentially removed at 3-h intervals for ana lysis. From 285 oocytes matured under these conditions, 114 (40.0%) de veloped to blastocysts on Day 9 after fertilization. Gelatin zymograms revealed protease activity at about 55 kDa for COCs matured for at le ast 3 h and two additional proteolytic zones at about 70 kDa after at least 9 h of IVM. The mRNAs of ECM-degrading components urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor 1 (PAI1) , matrix metalloproteinase 1 (MMP1), and tissue inhibitor of metallopr oteinases 1 (TIMP1), as well as TGF beta 1, bFGF, and FGFR, were detec ted during IVM in a factor-specific pattern: all transcript levels fou nd at COC 0 generally increased after 3 h of maturation and either rem ained high or decreased thereafter. On the basis of the chosen reverse transcription-polymerase chain reaction technique, one could suggest relative higher mRNA concentrations for TIMP1, PAI1, and both growth f actors compared to uPA, MMP1, and FGFR. Our results suggest a finely t uned extracellular proteolysis during IVM of bovine COCs, for which th e concerted action of modulating growth factors like bFGF and TGF beta 1 may be essential.