LANGMUIR-BLODGETT-FILM OF HYDROGENASE FOR ELECTROCHEMICAL HYDROGEN-PRODUCTION

LB films of thermostable hydrogenase from Thiocapsa roseopersicina wer e prepared on electrodes. Poly-L-lysine was used as a counter ion whic h stabilizes the protein and improves the protein transfer onto substr ates. Both hydrogen production and oxidation activities were observed in the hydrogenase monolayer on substrate. The monolayer film of hydro genase showed a high thermal stability as in solution, i.e. denaturati on did not occur up to 70 degrees C. Electrochemical hydrogen evolutio n was observed by biasing the ITO electrode on which the LB film of hy drogenase was mounted in the presence of methyl viologen as a mediator . (C) 1998 Elsevier Science S.A. All rights reserved.