IN-SITU QUARTZ-CRYSTAL MICROBALANCE MONITORING OF FAB'-FRAGMENT BINDING TO LINKER LIPIDS IN A PHOSPHATIDYLCHOLINE MONOLAYER MATRIX - APPLICATION TO IMMUNOSENSORS

Linker lipids were embedded in a phosphatidylcholine monolayer matrix prepared at the air-water interface. The covalent coupling of antibody fragments, non-specific adsorption of bovine serum albumin and specif ic binding of antibodies was monitored in situ with a 10-MHz quartz cr ystal microbalance. The attachment of antibody fragments and the activ ity of the layers was also showed with standardized radioimmunoassay. The results demonstrate that the coupling of Fab'-fragments to linker lipids in a monolayer matrix is a promising approach to achieve a high ly oriented layer of antibody fragments with a high density of binding sites on the sensor surface for immunological measurements. (C) 1998 Elsevier Science S.A. All rights reserved.