ENZYMATIC-SYNTHESIS OF BETA-GLUCURONIDES OF ESTRADIOL, ETHYNYLESTRADIOL AND OTHER PHENOLIC SUBSTRATES ON A PREPARATIVE-SCALE EMPLOYING UDP-GLUCURONYL TRANSFERASE

By incubation of bovine liver UDP-glucuronyl transferase with various phenolic aglycone substrates, preparation of the corresponding beta-gl ucuronides could be achieved in milligram quantities. Concomitantly, s imple access was generated to estrogen steroid glucuronides of great b iological importance, In terms of experimental feasibility, direct app lication of the commercially available crude microsomal product was co mpared to and proven to be competitive with the usually preferred util ization of an immobilized enzyme preparation.