ANALYSIS OF ROTENOIDS BY HIGH-TEMPERATURE HIGH-RESOLUTION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A short glass capillary column coated with PS-090 (20% phenyl-80% meth ylpolysiloxane, of 10 m x 0.30 mm i.d.; with 0.1 mu m film thickness) was used to analyze samples from Tephrosia candida; eighteen rotenoids were separated and identified without derivatization by HT-HRGC and H T-HRGC-MS. The mass spectra show the typical fragmentation pattern for rotenoids, with the base peaks either at M+, or originating from RDA (Retro Diels-Alder) rearrangements, HT-HRGC and HT-HRGC-MS were shown to be extremely valuable and neglected techniques for structural studi es as well as the routine analysis of rotenoids in crude extracts, Pos sible applications to other classes of aromatic natural products, e.g. flavonoids, are envisaged.