PANCREATIC LIPASE-RELATED PROTEIN-1 (PLRP1) IS PRESENT IN THE PANCREATIC-JUICE OF SEVERAL SPECIES

Pancreatic lipase-related protein 1 (PLRP1) was purified from human, c anine, porcine and rat pancreatic juices. The four PLRPls were identif ied using microsequencing methods after performing gel filtration on U ltrogel AcA-54 followed by chromatography on Heparin-Sepharose cation- exchanger. Polyclonal antibodies specific to human PLRP1 (HPLRP1) were raised in the rabbit using a synthetic decapeptide from HPLRP1. The r esults of Western blotting analysis showed that these antibodies recog nized native HPLRP1 and recombinant HPLRP1 produced by insect cells, a nd cross-reacted only with rat PLRP1 (RPLRP1). No significant lipolyti c activity was observed with native canine PLRP1 and recombinant HPLRP 1 on various glycerides, phospholipid and vitamin esters, or on choles terol esters. It was established for the first time that this protein is secreted in variable amounts by the adult exocrine pancreas of seve ral species. (C) 1998 Elsevier Science B.V. All rights reserved.