A VECTOR FOR THE EXPRESSION OF RECOMBINANT MONOCLONAL FAB FRAGMENTS IN BACTERIA

The availability of genes coding for monoclonal Fab fragments of a des ired specificity permits their expression in bacteria and provides a s imple method for the generation of good quality reagents. in this pape r we describe a new phagemid vector for the production of recombinant Fabs from penes obtained from phage display combinatorial libraries. T he phagemid features an antibiotic resistance cassette which: once ins erted between the heavy chain fragment and the light chain genes, avoi ds unwanted recombination and preserves useful restriction sites not a ffecting the Fab production rate. (C) 1998 Elsevier Science B.V. All r ights reserved.