ADDUCT DISTRIBUTIONS IN PISCINE DNA - SOUTH-EASTERN BLACK-SEA

The south-eastern Black Sea is relatively unpolluted and contains poly nuclear aromatic hydrocarbons (PAHs) only of the order of 1 mu g l(-1) . Pelagic and benthic fish were monitored during three successive wint ers from 1993 to 1996 for PAHs and for DNA adducts, The fish caught (M ugil sp,, Mullus barbatus, Alosa fallax, Merlangius merlangus, Sprattu s sprattus and Platichthys flesus) varied annually in length, weight a nd species composition, Although their livers were oily and contained significant concentrations of PAHs (11-56 mu g g(-1)), P-32-postlabell ing relatively low levels of hydrophobic DNA adducts (3-20 per 10(8) n ucleotides) in either fish livers or blood. A significant fraction of the DNA adducts in the blood samples, unlike those in the livers, were dephosphorylated by nuclease-P1. (C) 1998 Elsevier Science Ltd. All r ights reserved.