CHANGES IN SERUM IMMUNOGLOBULIN-M (IGM) CONCENTRATIONS DURING EARLY DEVELOPMENT OF CHUM SALMON (ONCORHYNCHUS-KETA) AS DETERMINED BY SENSITIVE ELISA TECHNIQUE
M. Nagae et al., CHANGES IN SERUM IMMUNOGLOBULIN-M (IGM) CONCENTRATIONS DURING EARLY DEVELOPMENT OF CHUM SALMON (ONCORHYNCHUS-KETA) AS DETERMINED BY SENSITIVE ELISA TECHNIQUE, Comparative biochemistry and physiology. Part A, Physiology, 106(1), 1993, pp. 69-74
1. A specific and sensitive sandwich enzyme-linked immunosorbent assay
(ELISA) was developed for the measurement of low levels of serum immu
noglobulin M (IgM) of chum salmon Oncorhynchus keta. 2. In this assay,
5 mul serum was enough to measure the concentration of IgM and the mi
nimum detectable concentration of serum IgM was about 5 ng/ml. 3. Coef
ficients of variation within and between assays ranged from 2.90 to 9.
61%. 4. IgM concentrations remained at low level (< 300 ng/ml) until 4
0 days after hatching and then increased rapidly at the period of emer
gence (48 days after hatching).