NITROGEN-FIXATION CAPACITY AND NODULE OCCUPANCY BY BRADYRHIZOBIUM-JAPONICUM AND B-ELKANII STRAINS

In a previous study soybean Bradyrhizobium strains, used in Brazilian studies and inoculants over the last 30 years, and strains adapted to the Brazilian Cerrados, a region frequently submitted to environmental and nutritional stresses, were analyzed for 32 morphological and phys iological parameters in vivo and in vitro. A cluster analysis allowed the subdivision of these strains into species Bradyrhizobium japonicum Bradyrhizobium elkanii and a mixed genotype. In this study, the bacte ria were analyzed for nodulation, N-2 fixation capacity, nodule occupa ncy and the ability to increase yield. The goal was to find a relation ship between the strain groups and the symbiotic performance. Two stra ins of Brazilian B. japonicum showed higher rates of N-2 fixation and nodule efficiency (mg of N mg(-1) of nodules) under axenic conditions. These strains also showed greater yield increases in field experiment s when compared to B. elkanii strains. However, no differences were de tected between B. japonicum and B. elkanii strains when comparing nodu le occupancy capacity. The adapted strains belonging to the serogroup B. elkanii SEMIA 566, most clustered in a mixed genotype, were more co mpetitive than the parental strain, and some showed a higher capacity of N-2 fixation. Some of the adapted strains, such as S-370 and S-372, have shown similar N-2 fixation rates and nodulation competitiveness to two Brazilian strains of B. japonicum. This similarity demonstrates the possibility of enhancing N-2 fixing ability, after local adaptati on, even within B. elkanii species. Differences in the DNA profiles we re also detected between the parental SEMIA 566 and the adapted strain s by analyses with the ERIC and REP-PCR techniques. Consequently. gene tic, morphological and physiological changes can be a result of adapta tion of rhizobia to the soil. This variability can be used to select s trains capable of increasing the contribution of N-2 fixation to soybe an nutrition.