STRUCTURAL-FUNCTIONAL STATE OF LYMPHOCYTE MEMBRANES FROM PERIPHERAL-BLOOD OF PATIENTS SUFFERING FROM SYSTEMIC LUPUS-ERYTHEMATOSUS AND RHEUMATOID-ARTHRITIS
Vm. Mazhul et al., STRUCTURAL-FUNCTIONAL STATE OF LYMPHOCYTE MEMBRANES FROM PERIPHERAL-BLOOD OF PATIENTS SUFFERING FROM SYSTEMIC LUPUS-ERYTHEMATOSUS AND RHEUMATOID-ARTHRITIS, Doklady Akademii nauk BSSR, 42(3), 1998, pp. 72-76
Structural-fuctional state of membrane proteins in peripheral blood ly
mphocytes was studied by millisecond room-temperature tryptophan phosp
horescence (RTTP) method in 43 patients with systemic lupus erythemato
sus (SLE) and rheumatoid arthritis (RA) and in 48 healthy people. A si
gnificant fall in lifetimes of the fast;Ind slow RTTP components of pa
tient lymphocytes was revealed. II provides evidence of reduced rigidi
ty in the microenvironment of membrane proteins chromophors leading to
enhanced intramolecular dynamics. Pronounced structural shifts in lym
phocyte membranes correlated with clinical and immunological manifesta
tions of both pathologies. RTTP parameters are qualitatively new indic
es of membrane state in immunocompetent cells contributing to the gene
ral membrane protein pattern rather. than individual receptors and com
plementing tests characterizing immunopathological process in case of
SLE and RA.