SIZE SEPARATION OF SODIUM DODECYL-SULFATE COMPLEXES OF HUMAN PLASMA-PROTEINS BY CAPILLARY-ELECTROPHORESIS EMPLOYING LINEAR POLYACRYLAMIDE AS A SIEVING POLYMER

Electrophoretic conditions to separate sodium dodecyl sulfate (SDS) co mplexes of human plasma proteins according to their size differences, by capillary electrophoresis employing linear polyacrylamide as a siev ing matrix (LPA-CE), have been examined. Using the optimized separatio n conditions,SDS complexes of human plasma proteins not treated with r educing agents were separated into about 40 peaks and shoulders within 60 min. The molecular glass values of major peaks in a separation pat tern were estimated from a plot of molecular mass and migration time f or standard proteins and some of the major plasma proteins have been i dentified on the pattern. The electrophoretic conditions were successf ully applied for the analysis of proteins in immunoglobulin G (IgG) my eloma sera.