SIZE SEPARATION OF SODIUM DODECYL-SULFATE COMPLEXES OF HUMAN PLASMA-PROTEINS BY CAPILLARY-ELECTROPHORESIS EMPLOYING LINEAR POLYACRYLAMIDE AS A SIEVING POLYMER
T. Manabe et al., SIZE SEPARATION OF SODIUM DODECYL-SULFATE COMPLEXES OF HUMAN PLASMA-PROTEINS BY CAPILLARY-ELECTROPHORESIS EMPLOYING LINEAR POLYACRYLAMIDE AS A SIEVING POLYMER, Electrophoresis, 19(13), 1998, pp. 2308-2316
Citations number
22
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Electrophoretic conditions to separate sodium dodecyl sulfate (SDS) co
mplexes of human plasma proteins according to their size differences,
by capillary electrophoresis employing linear polyacrylamide as a siev
ing matrix (LPA-CE), have been examined. Using the optimized separatio
n conditions,SDS complexes of human plasma proteins not treated with r
educing agents were separated into about 40 peaks and shoulders within
60 min. The molecular glass values of major peaks in a separation pat
tern were estimated from a plot of molecular mass and migration time f
or standard proteins and some of the major plasma proteins have been i
dentified on the pattern. The electrophoretic conditions were successf
ully applied for the analysis of proteins in immunoglobulin G (IgG) my
eloma sera.