THE RELATIONSHIP BETWEEN THE REGENERATION SYSTEM AND GENETIC-VARIABILITY IN THE CUCUMBER (CUCUMIS-SATIVUS L.)

Somaclonal variation in the Borszczagowski line of Cucumis sativus L. was determined for five regeneration systems: micropropagation (MP), d irect leaf callus regeneration (DLR), leaf callus regeneration (LCR), recurrent leaf callus regeneration (RLCR), and direct protoplast regen eration (DPR). The frequency at which new phenotypes appeared in R-1 l ines and the stability of the rDNA region analysed using of five probe s were investigated. MP was not subject to change, while DLR caused on ly infrequent changes. The highest frequency of change arose through D PR (90% of lines) and RLCR (42.8%), as opposed to 5.9% with LCR. Tetra ploids were produced only in the case of LCR (4.7%) and RLCR (28%).