Gm. Xia et al., ASYMMETRIC SOMATIC HYBRIDIZATION BETWEEN HAPLOID COMMON WHEAT AND UV-IRRADIATED HAYNALDIA-VILLOSA, PLANT SCI, 137(2), 1998, pp. 217-223
UV irradiated protoplasts from young embryo derived calli of Haynaldia
villosa were fused with protoplasts originated from anther derived ca
lli of Triticum aestivum cv. 24-2 by the PEG method. Regenerated calli
from fusion cells were obtained but none of them differentiated into
plants. The cell clones that formed early were examined for their hybr
id nature, chromosome counting and isozyme analysis suggested that the
y were nuclear hybrid. Further unequivocal confirmation was accomplish
ed using the technique of in situ hybridization (ISH) and RAPD analysi
s, which facilitated the identification of the hybrid nature in fusion
products. The results revealed that UV might have played an important
role in breaking donor chromosomes and ensuring the introduction of a
lien chromatin into the recipient via asymmetric hybridization. The fa
ilure of plant regeneration of the somatic hybrids was discussed and f
urther investigations are being conducted. (C) 1998 Elsevier Science I
reland Ltd. All rights reserved.