VARIABILITY IN 6 PEA GENE-SEQUENCES AND MAPPING THROUGH PCR-BASED MARKERS

Despite the rapid increase in sequence databases, gene sequences are s till under-used in the plant breeding and genetic mapping area. This s tudy was conducted to determine whether pea gene sequences contained e nough polymorphism to be used as genetic markers. Molecular variabilit y was examined at the DNA sequence level within different lines and wi ld ecotypes of Pisum sativum. The analysis was conducted for several i ntrons, exons and 5'UTR sequences from six nuclear genes (GAPC, PHYA a nd IAA-related genes). Each region was specifically amplified and poly morphism was identified by electrophoretic mobility and by direct sequ encing of PCR products. The observed polymorphism illustrates the poss ibility of developing molecular markers since all the analyzed loci ha ve been successfully localised. Polymorphism was detected either as DN A conformational polymorphism following non-denaturing polyacrylamide gel electrophoresis or as CAPS (cleaved amplified polymorphism sequenc e). The noteworthy property of such genetic markers is their ability t o establish bridges between different existing pea genetic maps. ((C) Inra/Elsevier, Paris.).