STRUCTURE-SPECIFIC DNA CLEAVAGE BY 5' NUCLEASES

Authors
CESKA TA SAYERS JR
Citation
Ta. Ceska et Jr. Sayers, STRUCTURE-SPECIFIC DNA CLEAVAGE BY 5' NUCLEASES, Trends in biochemical sciences, 23(9), 1998, pp. 331-336
Citations number
43
Categorie Soggetti
Biology
Journal title
Trends in biochemical sciences → ACNP
ISSN journal
09680004
Volume
23
Issue
9
Year of publication
1998
Pages
331 - 336
Database
ISI
SICI code
0968-0004(1998)23:9<331:SDCB5N>2.0.ZU;2-M
Abstract
Many processes, such as DNA replication, recombination and repair, pro duce branched DNA structures. These bifurcated molecules are trimmed b y a group of homologous 5'-3' exonucleases (also known as 5' nucleases ) in structure-specific cleavage reactions. X-ray crystallographical a nd biochemical studies suggest that ssDNA substrates become threaded t hrough the 5'-3' exonucleases, where hydrolysis is effected with the a id of divalent metal cations.