The risk of transfusion-transmitted viral infections may be estimated
by several methods, but only prospective studies of transfusion recipi
ents can directly measure the incidence, with associated 95% upper con
fidence bound, of these infections. From 1989 through 1995, 764 recipi
ents of allogeneic or autologous red blood cell transfusions were enro
lled; 486 (64%) provided both pretransfusion and 6-month follow-up spe
cimens. Both specimens were tested for anti-HBc, anti-HCV, anti-HTLV-I
and anti-HIV, with appropriate confirmatory testing. Thirty-nine (8.0
%) subjects had seroprevalent anti-HBc, 19 (3.9%) subjects had seropre
valent anti-HCV, three (0.6%) subjects had seroprevalent anti-HTLV-I/I
I, and one (0.2%) subject had seroprevalent anti-HIV. There were no se
roconversions for any agent among the 34 patients who received only au
tologous blood, and no confirmed seroconversions for anti-HTLV-I or an
ti-HIV among all subjects. There were three seroconversions for anti-H
Bc (incidence 1.04 x 10(-3). 95% confidence interval (Ci) 2.15 x 10(-4
), 3.05 x 10(-3) per allogeneic unit transfused), and two confirmed se
roconversions for HCV (incidence 6.94 x 10(-4); 95% CI 8.34 x 10(-5),
2.51 x 10(-3) per allogeneic unit transfused). One of the two anti-HCV
seroconversions occurred in March 1994, after the institution of HCV
EIA 2.0 screening of donated blood. Transfusion-associated seroconvers
ions to hepatitis B and C markers were observed at low rates in the ea
rly 1990s despite testing donors for markers of both viruses, whereas
seroconversions to HTLV-I or HIV were less than 1.04 x 10(3) per allog
eneic unit transfused, based upon the upper 95% confidence interval of
the zero incidence in this study.