DEVELOPMENT OF A SIGNAL MEASUREMENT TECHNIQUE FOR APPLICATION IN MULTIANALYTE LIPOSOME IMMUNOMIGRATION ASSAYS

The potential for development of a multi-analyte liposome immunomigrat ion solid-phase competition (LIM-SPC) assay based on multivariate cali bration of spectral reflectance measurements was investigated. A simul ation of a multi-analyte LIM-SPC assay was carried out using a mixture of three analyte-tagged liposome preparations, each containing a diff erent colored dye. The liposome preparations were combined according t o a statistical experiment design and allowed to migrate up nitrocellu lose test strips to a binding zone. After the liposomes were captured at this Zone, the reflectance spectrum of the zone was measured. Parti al least squares regression was used to develop multivariate calibrati ons. For the liposome-encapsulated dyes, the coefficients of determina tion obtained for carboxyfluorescein, sulforhodamine B and isosulfan b lue were 0.902, 0.990 and 0.977, respectively. It was concluded that t he approach used was suitable for a multi-analyte LIM-SPC.