Experiments on acetotrophic methanogenesis were conducted at 37 degree
s C in a 3.5 l chemostat reactor and ten 120 ml batch reactors. The de
gradation process produced substantial quantities of soluble microbial
products (SMP). Converting 1 mg of acetate produced 0.0251 mg-C of SM
P in the chemostat reactor but 0.0150 mg-C of SMP In batch reactors. T
he reaction was not pH sensitive within the range of pH 6-8. At pH 5 a
nd 9, methanogenesis and SMP production were both inhibited. The bioma
ss inhibited at pH 9 was able to regain activities after adjusting the
pH to neutrality, but that inhibited at pH 5 was not. The biomass yie
ld was 0.0257 and 0.0297 mg-biomass/mg-acetate in chemostat and batch
reactors, respectively. (C) 1998 Elsevier Science Ltd. All rights rese
rved.